Coding

Part:BBa_K2872998:Design

Designed by: Dina Altarawneh   Group: iGEM18_CMUQ   (2018-10-10)


Cas12a protein fused with NLS and HA tag at C-terminus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1894
    Illegal PstI site found at 2392
    Illegal PstI site found at 4099
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 657
    Illegal NheI site found at 5091
    Illegal PstI site found at 1894
    Illegal PstI site found at 2392
    Illegal PstI site found at 4099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1634
    Illegal BglII site found at 1967
    Illegal BglII site found at 2537
    Illegal BglII site found at 2804
    Illegal BglII site found at 3425
    Illegal BamHI site found at 4437
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1894
    Illegal PstI site found at 2392
    Illegal PstI site found at 4099
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1894
    Illegal PstI site found at 2392
    Illegal PstI site found at 4099
    Illegal NgoMIV site found at 1696
    Illegal NgoMIV site found at 2695
    Illegal NgoMIV site found at 3397
    Illegal NgoMIV site found at 4394
    Illegal NgoMIV site found at 4413
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644
    Illegal BsaI.rc site found at 5078
    Illegal SapI.rc site found at 2025
    Illegal SapI.rc site found at 2085


Design Notes

none


Source

none

References